During which phase of mitosis do sister chromatids align at the center of the cell?

During metaphase, sister chromatids align at the cell's equatorial plane, also known as the metaphase plate. This alignment is critical because it ensures that, when the cell proceeds to the next phase, each sister chromatid will be pulled apart into separate daughter cells. The spindle fibers extend from the centrosomes to the kinetochores of the chromatids, securing them in place for an orderly separation. This precise arrangement minimizes the risk of chromosomal errors, which could lead to abnormalities in the daughter cells. Each sister chromatid remains attached at the centromere until they are separated later in anaphase. Understanding this alignment and its significance is crucial for comprehending the cell division process and ensuring genetic stability during cell replication.